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Flow Cytometry

Flow Cytometry is a rapidly developing and amazingly flexible technology that combines many of the advantages of microscopy and biochemical analysis to allow fast, sensitive and reproducible multiparametric analyses at the single cell level. It has introduced new horizons in the identification and characterization of phenotypically heterogeneous cell populations in a wide range of research areas. Cell Sorting is an advanced key technology in Flow Cytometry and a fundamental tool in medical research, which allows rapid analysis and accurate collection of specific cell populations at extremely high speed, high purity and high viability for further research or clinical purposes.

Staff

The Flow Cytometry facility is managed by Maggie (Xin) Wang.

Suat Dervish is the Flow Cytometry Scientist located at WMI.

Christine Smyth is the Flow Cytometry Scientist located at CMRI. 

Services

Cell Sorting services are available to researchers, utilising the two Cell Sorting instruments in the facility. Cell Sorting services are categorised as middle range or complex to reflect the level of capabilities available from the two different instruments.
 
The following table outlines the instruments used for each service:
 

Service

Instrument

Excitation

No. of fluorescent detectors

Acquisition software

Collection options

Sorting – Complex

BD InfluxTM  (including the small particle detection)

355nm, 405nm, 488nm, 561nm, 642nm

21

FACS Sortware v1

Up to 6 way sort, plate sort

Sorting – Middle range

BD AriaIIITM

405nm, 488nm, 640nm

10

FACSDiVa       v 6.1.3

Up to 4 way sort, plate sort


Technology

There are six Analytic Flow Cytometers available for use by trained researchers. The Analytic Flow Cytometers are also categorised as simple, intermediate or complex to reflect the level of analysis available from the different types of instruments.
 

Category

Instrument

Excitation

No. of fluorescent detectors

Acquisition software

Complex

BD FortessaTM

355nm, 405nm, 488nm, 561nm, 640nm

18

FACSDiVa      v 6.2

BD LSRIITM with HTS option

355nm, 405nm, 488nm, 561nm, 640nm

18

FACSDiVa      v 6.2

Intermediate

BD FACSCantoIITM _ #1

405nm, 488nm, 640nm

8

FACSDiVa      v 6.1.3

BD FACSCantoIITM _ #2

405nm, 488nm, 640nm

8

FACSDiVa      v 6.1.3

Simple

BD FACSCantoATM

488nm, 640nm

6

FACSDiVa      v 6.1.2

BC FC500TM

488nm, 637nm

5

CXP

 

Location

The Flow Cytometry facility is co-located between the Westmead Millennium Institute for Medical Research and the Children's Medical Research Institute.

 

The Flow Cytometry Facility at Westmead Research Hub is supported by a grant from the Cancer Institute of NSW.